How Do You Spell APACI ENDONUCLEASE?

1. ApaCI endonuclease is a type II restriction enzyme that is commonly used in molecular biology research. It is derived from the bacterium Arthrobacter species, specifically Arthrobacter protophormiae. This endonuclease recognizes a specific DNA sequence and cleaves it at a particular location, resulting in the generation of DNA fragments with cohesive or sticky ends.

   The recognition sequence for ApaCI endonuclease is five nucleotides long, 5'-5'CCTGCAGG-3', and the cleavage occurs between the two guanine nucleotides in this sequence, generating cohesive ends with a 4-nucleotide overhang: 5'-CCTG and G-3'. This specific cleavage pattern allows for the subsequent ligation of the DNA fragments into a vector or another DNA molecule with compatible cohesive ends. ApaCI recognition site and cleavage pattern are frequently utilized in DNA cloning techniques.

   ApaCI endonuclease is a highly specific enzyme, meaning it only cleaves DNA molecules that possess the exact recognition sequence. This characteristic ensures that the enzyme does not indiscriminately digest DNA in a complex mixture, providing researchers with precise targeted cleavage capabilities. Additionally, ApaCI endonuclease is generally heat-labile, which means it can be easily inactivated by heating the enzyme at an elevated temperature, making it a controllable tool in molecular biology experiments.

   In summary, ApaCI endonuclease is a type II restriction enzyme derived from Arthrobacter protophormiae that recognizes the DNA sequence 5'-CCTGCAGG-3' and cleaves it between two guanine nucleotides. Its specific DNA cleavage properties make it a valuable tool in molecular biology techniques such as

Common Misspellings for APACI ENDONUCLEASE

The word "ApaCI" is a combination of two parts: "Apa" and "CI". The "Apa" part is derived from the enzyme's source organism, which is Anabaena sp. strain PCC-7120. The "CI" portion indicates that it was the first endonuclease discovered from this organism.

In scientific nomenclature, enzymes are typically named after the organism they originated from, followed by an abbreviation indicating the specific enzyme. The term "endonuclease" refers to a type of enzyme that cleaves DNA or RNA at specific sites within the nucleic acid sequence.

Therefore, "ApaCI Endonuclease" can be understood as the endonuclease enzyme derived from Anabaena sp. strain PCC-7120, and it is one among several discovered enzymes from this organism.