DEOXYRIBONUCLEASE BSTFI Meaning and
Definition
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Deoxyribonuclease BstFI is an enzyme that belongs to the family of restriction endonucleases. This enzyme is derived from the bacterium Bacillus stearothermophilus and is widely used in molecular biology research to cut DNA molecules at specific recognition sites. The name "BstFI" is derived from the bacterial species and strain it was originally isolated from, and the Roman numeral "I" indicates that it is the first restriction endonuclease isolated from this strain.
Deoxyribonuclease BstFI recognizes a specific DNA sequence and cleaves the DNA molecule at a defined site within that sequence. This enzyme specifically recognizes the palindromic DNA sequence 5'-CGCG-3' and cuts the DNA between the two cytosine residues. The resulting DNA fragments have overhanging ends, known as sticky ends, which can be useful for further molecular biology applications such as DNA cloning or gene manipulation.
The activity of Deoxyribonuclease BstFI is dependent on several factors, including the concentration of the enzyme, reaction conditions (e.g., temperature, ionic strength), and the presence of cofactors. This enzyme has a preference for Mg2+ ions as a cofactor, which enhances its catalytic activity.
Overall, Deoxyribonuclease BstFI is a valuable tool in molecular biology research for the precise manipulation of DNA molecules. Its ability to recognize and cut DNA at specific sites makes it an essential enzyme in various experimental techniques, including DNA sequencing, gene cloning, and genetic engineering.
Common Misspellings for DEOXYRIBONUCLEASE BSTFI
- seoxyribonuclease bstfi
- xeoxyribonuclease bstfi
- ceoxyribonuclease bstfi
- feoxyribonuclease bstfi
- reoxyribonuclease bstfi
- eeoxyribonuclease bstfi
- dwoxyribonuclease bstfi
- dsoxyribonuclease bstfi
- ddoxyribonuclease bstfi
- droxyribonuclease bstfi
- d4oxyribonuclease bstfi
- d3oxyribonuclease bstfi
- deixyribonuclease bstfi
- dekxyribonuclease bstfi
- delxyribonuclease bstfi
- depxyribonuclease bstfi
- de0xyribonuclease bstfi
- de9xyribonuclease bstfi
- deozyribonuclease bstfi
- deocyribonuclease bstfi
Etymology of DEOXYRIBONUCLEASE BSTFI
The word "Deoxyribonuclease BstFI" can be broken down as follows:
1. Deoxyribonuclease: The term "deoxyribonuclease" is derived from combining three components:
- Deoxyribose: referring to the sugar found in DNA molecules.
- Nucleo-: indicating a relationship with nucleic acids (such as DNA).
- -ase: denoting an enzyme (a protein that catalyzes a chemical reaction).
Therefore, "deoxyribonuclease" refers to an enzyme that breaks down DNA molecules.
2. BstFI: This portion refers to a specific enzyme, whose name is an acronym.
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