How Do You Spell ENDONUCLEASE NASBI?

1. Endonuclease NasBI is a type II restriction endonuclease that is commonly found in bacteria. It is derived from the bacterium Nostoc sp. Strain PCC7120 and belongs to the PD-D/EXK superfamily of restriction enzymes.

   Endonuclease NasBI is an enzyme that is capable of recognizing specific DNA sequences and cleaving the phosphodiester bonds within the DNA molecule, resulting in the fragmentation of the DNA. It recognizes and cleaves the DNA sequence 5'-TGCA-3', specifically at the position where the adenine residue is located on the top strand and the guanine residue is located on the bottom strand. This results in the production of blunt DNA ends with a 4-base 5' overhang.

   This endonuclease is often used in molecular biology research and biotechnology applications as a tool for DNA analysis and manipulation. It is especially useful in the field of genetic engineering as it allows for the precise cleavage of DNA at specific sites, enabling the insertion, deletion, or modification of specific DNA segments.

   The function of Endonuclease NasBI in bacteria is thought to be a defense mechanism against foreign DNA, such as viral and plasmid DNA. By cleaving these foreign DNA molecules, the bacterium can protect its own genome from potential harm and maintain genetic stability.

   In summary, Endonuclease NasBI is a type II restriction endonuclease derived from Nostoc sp. Strain PCC7120 that specifically recognizes and cleaves the DNA sequence 5'-TGCA-3', providing useful tools for DNA analysis and genetic engineering applications.

Common Misspellings for ENDONUCLEASE NASBI

The word "endonuclease" originates from the combination of two Greek terms: "endon" meaning "within" and "nuclease" referring to an enzyme that breaks down nucleic acids (DNA or RNA). Therefore, "endonuclease" literally means an enzyme that cleaves within nucleic acids.

The term "NasBI" is not a commonly used word outside of the scientific community. It is a specific name given to a type II restriction endonuclease, which is an enzyme that recognizes and cleaves DNA at specific nucleotide sequences. The name "NasBI" is an abbreviation of its strain name and serves to distinguish this particular endonuclease from others.

In summary, "Endonuclease NasBI" indicates an endonuclease enzyme that cleaves within nucleic acids and is specifically referred to as "NasBI" due to its strain name or classification.