RESTRICTION ENDONUCLEASE MAPPING Meaning and
Definition
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Restriction endonuclease mapping is a technique used in molecular biology to determine the sequence and location of restriction enzyme recognition sites within a DNA molecule. Restriction endonucleases are enzymes that recognize specific DNA sequences, known as restriction sites, and cleave the DNA at those sites. This technique combines the principles of restriction digestion and gel electrophoresis to create a DNA map.
The process involves several steps. Firstly, the DNA of interest is isolated and purified. Then, a specific restriction endonuclease is chosen that recognizes a specific DNA sequence within the target DNA molecule. The target DNA is then incubated with the restriction enzyme, resulting in the cleavage of the DNA at the recognition sites.
The next step involves size separation of the resulting DNA fragments using agarose gel electrophoresis. The fragments are loaded onto the gel and an electric field is applied, causing the DNA fragments to migrate through the gel matrix. The smaller fragments migrate more quickly than larger ones, allowing for size separation.
After separation, the DNA fragments are visualized using staining agents, such as ethidium bromide or fluorescent dyes, and a UV light source. The resulting pattern of DNA bands represents the distribution and size of the fragments produced by the restriction enzyme digestion. By comparing this pattern with known DNA standards or markers, the location and sequence of the restriction sites within the target DNA can be determined and mapped. This mapping technique has numerous applications, such as genetic mapping, gene cloning, and DNA fingerprinting.
Common Misspellings for RESTRICTION ENDONUCLEASE MAPPING
- eestriction endonuclease mapping
- destriction endonuclease mapping
- festriction endonuclease mapping
- testriction endonuclease mapping
- 5estriction endonuclease mapping
- 4estriction endonuclease mapping
- rwstriction endonuclease mapping
- rsstriction endonuclease mapping
- rdstriction endonuclease mapping
- rrstriction endonuclease mapping
- r4striction endonuclease mapping
- r3striction endonuclease mapping
- reatriction endonuclease mapping
- reztriction endonuclease mapping
- rextriction endonuclease mapping
- redtriction endonuclease mapping
- reetriction endonuclease mapping
- rewtriction endonuclease mapping
- resrriction endonuclease mapping
- resfriction endonuclease mapping
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