TRIS BUFFER Meaning and
Definition
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Tris buffer, short for Tris(hydroxymethyl)aminomethane buffer, is a type of biological buffer commonly used in biochemical and molecular biology experiments to control and maintain a stable pH level in a solution. It is an amino-containing organic compound that acts as a weak base and is often used in combination with its conjugate acid, Tris-HCl, to create a pH buffer system.
Tris buffer is highly advantageous due to its compatibility with many biochemical processes and its resistance to pH changes when diluted or in the presence of biological molecules. It has a pH range of approximately 7 to 9, making it suitable for a wide range of experimental conditions. The buffer capacity of Tris buffer makes it useful in maintaining the desired pH level, thus ensuring the optimal functioning of enzymes and other biological molecules that are sensitive to pH changes.
Tris buffer is prepared by dissolving Tris base in deionized water, followed by titration with hydrochloric acid (HCl) to reach the desired pH. The concentration of Tris buffer is typically around 10 to 100 mM, depending on the specific experimental requirements.
Overall, Tris buffer is a widely used tool in biological research, providing researchers with a reliable means to control and maintain optimal pH conditions, ultimately enhancing the accuracy and reproducibility of their experimental results.
Common Misspellings for TRIS BUFFER
- rris buffer
- fris buffer
- gris buffer
- yris buffer
- 6ris buffer
- 5ris buffer
- teis buffer
- tdis buffer
- tfis buffer
- ttis buffer
- t5is buffer
- t4is buffer
- trus buffer
- trjs buffer
- trks buffer
- tros buffer
- tr9s buffer
- tr8s buffer
- tria buffer
- triz buffer
Etymology of TRIS BUFFER
The word "Tris" in "Tris buffer" is derived from the chemical compound tris(hydroxymethyl)aminomethane. Tris is a common laboratory reagent utilized to prepare buffer solutions, which are used to maintain a stable pH in various biochemical and molecular biology experiments. The name "tris" is derived from the compound's chemical structure, which consists of three hydroxymethyl groups attached to a nitrogen atom. The abbreviation "Tris" is commonly used in scientific literature and protocols to refer to this compound and its related buffer solutions.
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