NITROTETRAZOLIUM BLUE Meaning and
Definition
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Nitrotetrazolium Blue (NBT) is a chemical compound that belongs to the class of tetrazolium salts. It is an organic compound with the molecular formula C40H30N10O10 and a molar mass of 868.73 grams per mole. NBT is typically used as a redox indicator in biochemical and histological studies.
NBT is a pale yellow, water-soluble powder that becomes blue in the presence of specific reducing agents. It is often used to visualize the location and activity of oxidoreductase enzymes within cells and tissues. NBT is reduced by these enzymes into an insoluble formazan dye, which appears as blue or purple precipitates under light microscopy. This reaction allows researchers to observe the distribution and intensity of enzyme activity.
Due to its ability to detect enzymatic activity, NBT has found applications in diverse fields such as immunohistochemistry, virology, and cell biology. It has been particularly useful in studying oxidative stress, cellular metabolism, and the evaluation of drug efficacy.
It is important to note that NBT can be toxic and should be handled with care. Its usage requires appropriate safety precautions, including the use of personal protective equipment. Additionally, the interpretation of NBT-stained samples may require experience and expertise in the relevant field.
Common Misspellings for NITROTETRAZOLIUM BLUE
- bitrotetrazolium blue
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Etymology of NITROTETRAZOLIUM BLUE
The word "Nitrotetrazolium" is derived from its chemical composition. "Nitro" refers to the presence of a nitro group (NO2-), "tetra" indicates the presence of four nitrogen atoms, and "zolium" is a suffix used in organic chemistry to denote a tetrazolium compound.
The word "Blue" simply describes the color of the compound.
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